NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Cloning higher plants from aseptically cultured tissues and cellsA review of aseptic culture methods for higher plants is presented, which focuses on the existing problems that limit or prevent the full realization of cloning plants from free cells. It is shown that substantial progress in clonal multiplication has been made with explanted stem tips or lateral buds which can be stimulated to produce numerous precocious axillary branches. These branches can then be separated or subdivided and induced to root in order to yield populations of genetically and phenotypically uniorm plantlets. Similarly, undifferentiated calluses can sometimes be induced to form shoots and/or roots adventitiously. Although the cell culture techniques required to produce somatic embryos are presently rudimentary, steady advances are being made in learning how to stimulate formation of somatic or adventive embryos from totipotent cells grown in suspension cultures. It is concluded that many problems exist in the producing and growing of totipotent or morphogenetically competent cell suspensions, but the potential benefits are great.
Document ID
19830030605
Document Type
Reprint (Version printed in journal)
Authors
Krikorian, A. D. (New York, State University Stony Brook, NY, United States)
Date Acquired
August 11, 2013
Publication Date
January 1, 1982
Publication Information
Publication: Biological Reviews
Volume: 57
Subject Category
LIFE SCIENCES (GENERAL)
Funding Number(s)
CONTRACT_GRANT: NSG-7270
Distribution Limits
Public
Copyright
Other