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Characteristics of statoliths from rootcaps and coleoptilesAmyloplasts with intact envelopes were isolated from corn coleoptiles, incubated with either cationized ferritin (CF) or fluorescein isothiocyanate (FITC)-CF, and examined with epifluorescence optics. Before incubation, the mean zeta potential was found to be -19.4 mv. The net negative surface charge was confirmed ultrastructurally by the binding of CF to both amyloplasts and starch grains. Binding to the latter was reduced compared to the former. FITC-CF showed binding to some but not all amyloplasts, and was absent from individual starch granules. Membrane-bound calcium, indicated by chlorotetracycline, fluorescence, was detected throughout the cytoplasm of root cap cells. These results may reflect an affinity of CF for membrane-based charge, and suggest that a calcium shell exists on the amyloplast envelope.
Document ID
19830046592
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Sack, F. D.
(Cornell Univ. Ithaca, NY, United States)
Leopold, A. C.
(Cornell University Ithaca, NY, United States)
Date Acquired
August 11, 2013
Publication Date
December 1, 1982
Subject Category
Life Sciences (General)
Accession Number
83A27810
Funding Number(s)
CONTRACT_GRANT: NAGW-3
Distribution Limits
Public
Copyright
Other

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