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Studies on aqueous two phase polymer systems useful for partitioning of biological materialsThe two phase systems that result when aqueous solutions of dextran and poly(ethylene glycol) (PEG) are mixed above a critical concentration of a few percent provide a useful medium for the separation of biological cell subpopulations via partition between the top, PEG-rich phase and the liquid-liquid phase boundary. Interfacial tensions of such systems have been measured by the rotating drop technique and found to range between 0.1-100 micro-N/m. The tension was found to depend on the length of the tie line describing the system on a phase diagram, via a power law relationship which differed depending on the concentration of Na phosphate buffer present. The electrokinetic properties of drops of one phase suspended in the other were studied for a variety of systems. It was found that the droplet electrophoretic mobility increased monotonically with phosphate concentration and drop diameter but exhibited the opposite sign from that anticipated from phosphate partition measurements. It was possible to take advantage of these electrokinetic properties and dramatically enhance the speed of phase separation through application of relatively small electric fields.
Document ID
19830062059
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Brooks, D. E.
(Oregon, University Portland, OR; British Columbia, University, Vancouver, Canada)
Bamberger, S.
(Oregon, University Portland, OR, United States)
Date Acquired
August 11, 2013
Publication Date
January 1, 1982
Subject Category
Inorganic And Physical Chemistry
Meeting Information
Meeting: Materials processing in the reduced gravity environment of space; Annual Meeting
Location: Boston, MA
Start Date: November 16, 1981
End Date: November 18, 1981
Sponsors: NASA
Accession Number
83A43277
Distribution Limits
Public
Copyright
Other

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