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Evaluation of the Separability of Monodisperse Polystyrene Latex Microspheres in a Continuous Flow Electrophoresis SystemThe continuous flow electrophoresis system makes electrophoresis possible in a free flowing film of aqueous electrolyte medium. The sample is introduced at one end of the chamber and is subjected to a lateral dc field. This process separates the sample into fractions since each component has a distinctive electrophoric mobility. Evaluations were made of sample conductivity and buffer conductivity as they affect sample band spread and separation using the Continuous Particle Electrophoresis (CPE) system. Samples were prepared from mixtures of 5 percent and 1 percent polystyrene latex (PSL) microspheres which were .4, .56 and .7 microns in diameter. These were prepared in electrolyte media 1x and 3x the conductivity of the curtain buffer, approximately 150 and 450 micro mhos/cm. Samples with matched conductivities produced greater resolution and less band spread than those with 3x the conductivity of the curtain buffer.
Document ID
19840007989
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Williams, G., Jr.
(Calhoun Community Coll. Decatur, AL, United States)
Date Acquired
August 12, 2013
Publication Date
December 1, 1983
Publication Information
Publication: Alabama Univ. Res. Rept.: 1983 NASA(ASEE Summer Faculty Fellowship Program
Subject Category
Inorganic And Physical Chemistry
Accession Number
84N16057
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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