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Presynaptic elements involved in the maintenance of the neuromuscular junctionAlterations in the neuromuscular junction were observed in rats preceding loss of muscle mass. In view of the possibility that these alterations involve changes in the secretion of myotrophic agents by presynaptic motor neurons, an investigation was undertaken to characterize a neuronall factor which is thought to be involved in the initiation and maintenance of cholinergic synapses. This factor, which is secreted into the incubation medium by NG108-15 neuroblastoma x glioma hybrid cells, induces the aggregation of nicotinic acetylcholine receptors on primary cultures of rat hindlimb myotubes. Previous attempts to purify this factor failed. Extensive washing of the NG108-15 cells with hepes-buffered salt solution followed by short (4 hour) collection times resulted in the collection of incubation medium containing maximal aggregation activity with as little as 5 ug secreted protein per ml of fresh medium. A three-fold increase in specific activity was obtained after anion exchange chromatography.
Document ID
19840010807
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Burrows, G. H.
(National Inst. of Mental Health Bethesda, MD, United States)
Date Acquired
August 12, 2013
Publication Date
February 1, 1984
Publication Information
Publication: NASA. Washington NASA Space Biol. Program
Subject Category
Life Sciences (General)
Accession Number
84N18875
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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