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Identification of a volatile phytotoxin from algaeThe objectives were to develop a trap system for isolating fractions of volatile algal phytotoxin and to characterize the major components of the isolated phytotoxin fractions. A bioassay using Phaseolus vulgaris seedlings was developed to aid in investigating the properties of the phytotoxin produced by cultures of Euglena gracilis var. bacillaris and Chlorella vulgaris. Two traps were found, 1.0 M hydrochloric acid and 0 C, which removed the phytotoxin from the algal effluent and which could be treated to release that phytotoxin as judged with the bioassay procedure. It was also determined that pretraps of 1.0 M sodium hydroxide and 1.0 M potassium biocarbonate could be used without lowering the phytotoxin effect. Ammonia was identified in trap solutions by ninhydrin reaction, indophenol reaction and derivatization with dansyl chloride and phenylisothiocyanate. Ammonia at the gaseous concentrations detected was found to have the same effects in the bioassay system as the volatile phytotoxin. It is possible that other basic, nitrogen containing compounds which augment the effects of ammonia were present at lower concentrations in the algal effluent.
Document ID
Document Type
Conference Paper
Garavelli, J. S. (Texas A&M Univ. College Station, TX, United States)
Fong, F. (Texas A&M Univ. College Station, TX, United States)
Funkhouser, E. A. (Texas A&M Univ. College Station, TX, United States)
Date Acquired
August 12, 2013
Publication Date
February 1, 1984
Publication Information
Publication: NASA. Washington NASA Space Biol. Program
Subject Category
Funding Number(s)
Distribution Limits
Work of the US Gov. Public Use Permitted.

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IDRelationTitle19840010761Analytic PrimaryNASA Space Biology Program. Eighth annual symposium's program and abstracts