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Cellular localization of Na(+), K(+)-ATPase in the mammalian vestibular systemTwo different, but complementary, procedures for cellular localization of Na+, K+-ATPase in the guinea pig vestibular system were employed. One of these techniques, devised by Stirling, depends upon the well documented ability of the specific inhibitor ouabain to bind selectively to Na+,K+-ATPase, blocking catalytic activity. Microdisected vestibular tissues are incubated with tritium-labelled (3H-) ouabain, and regions with a high concentration of Na+,K+-ATPase are subsequently identified by light microscope autoradiography. A second method, originated by Ernst, detects inorganic phosphate released from an artificial substrate (nitrophenyl phosphate) by catalytic activity of the enzyme. In the presence of strontium ion, phosphate is precipitated near regions of high activity, then converted to a product which may finally be visualized in the electron microscope. This cytochemical enzymatic reaction is inhibited by ouabain.
Document ID
19840010812
Document Type
Conference Paper
Authors
Kerr, T. P. (Michigan Univ. Ann Arbor, MI, United States)
Date Acquired
August 12, 2013
Publication Date
February 1, 1984
Publication Information
Publication: NASA. Washington NASA Space Biol. Program
Subject Category
LIFE SCIENCES (GENERAL)
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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