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Purification of biomaterials by phase partitioningA technique which is particularly suited to microgravity environments and which is potentially more powerful than electrophoresis is phase partitioning. Phase partitioning is purification by partitioning between the two immiscible aqueous layers formed by solution of the polymers poly(ethylene glycol) and dextran in water. This technique proved to be very useful for separations in one-g but is limited for cells because the cells are more dense than the phase solutions thus tend to sediment to the bottom of the container before reaching equilibrium with the preferred phase. There are three phases to work in this area: synthesis of new polymers for affinity phase partitioning; development of automated apparatus for ground-based separations; and design of apparatus for performing simple phase partitioning space experiments, including examination of mechanisms for separating phases in the absence of gravity.
Document ID
19850002735
Document Type
Conference Paper
Authors
Harris, J. M. (Alabama Univ. Huntsville, AL, United States)
Date Acquired
August 12, 2013
Publication Date
October 1, 1984
Publication Information
Publication: NASA. Marshall Space Flight Center 2nd Symp. on Space Industrialization
Subject Category
ASTRONAUTICS (GENERAL)
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.