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Effects of coating rectangular microscopic electrophoresis chamber with methylcelluloseOne of the biggest problems in obtaining high accuracy in microscopic electrophoresis is the parabolic flow of liquid in the chamber due to electroosmotic backflow during application of the electric field. In chambers with glass walls the source of polarization leading to electroosmosis is the negative charge of the silicare and other ions that form the wall structure. It was found by Hjerten, who used a rotating 3.0 mm capillary tube for free zone electrophoresis, that precisely neutralizing this charge was extremely difficult, but if a neutral polymer matrix (formaldehyde fixed methylcellulose) was formed over the glass (quartz) wall the double layer was displaced and the viscosity at the shear plane increased so that electroosmotic flow could be eliminated. Experiments were designed to determine the reliability with which methylcellulose coating of the Zeiss Cytopherometer chamber reduced electroosmotic backflow and the effect of coating on the accuracy of cell electrophoretic mobility (EPN) determinations. Fixed rat erythrocytes (RBC) were used as test particles.
Document ID
19850023435
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Plank, L. D.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31748
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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