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Identification and quantitation of morphological cell types in electrophoretically separated human embryonic kidney cell culturesFour major cell types were identified by phase microscopy in early passage human embryonic kidney cell cultures. They are small and large epithelioid, domed, and fenestrated cells. Fibroblasts are also present in some explants. The percent of each cell type changes with passage number as any given culture grows. As a general rule, the fraction of small epithelioid cells increases, while the fraction of fenestrated cells, always small, decreases further. When fibroblasts are present, they always increase in percentage of the total cell population. Electrophoretic separation of early passage cells showed that the domed cells have the highest electrophoretic mobility, fibroblasts have an intermediate high mobility, small epithelioid cells have a low mobility, broadly distributed, and fenestrated cells have the lowest mobility. All cell types were broadly distributed among electrophoretic subfractions, which were never pure but only enriched with respect to a given cell type.
Document ID
19850023451
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Williams, K. B.
(Pennsylvania State Univ. University Park, PA, United States)
Kunze, M. E.
(Pennsylvania State Univ. University Park, PA, United States)
Todd, P. W.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31764
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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