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Urokinase production by electrophoretically separated cultured human embryonic kidney cellsUrokinase is a plasminogen activator found in urine. Relatively pure preparations have been tested in Europe, Japan and the United States for the treatment of deep vein thrombosis and other dangerous blood clots. Human embryonic kidney cell cultures have been found to produce urokinase at much higher concentrations, but less than 5% of the cells in typical cultures are producers. Since human diploid cells become senescent in culture the selection of clones derived from single cells will not provide enough material to be useful, so a bulk purification method is needed for the isolation of urokinase producing cell populations. Preparative cell electrophoresis was chosen as the method, since evidence exists that human embryonic cell cultures are richly heterogeneous with respect to electrophoretic mobility, and preliminary electrophoretic separations on the Apollo-Soyuz space flight produced cell populations that were rich in urokinase production. Similarly, erythropoietin is useful in the treatment of certain anemias and is a kidney cell duct, and electrophoretically enriched cell populations producing this product have been reported. Thus, there is a clear need for diploid human cells that produce these products, and there is evidence that such cells should be separable by free-flow cell electrophoresis.
Document ID
19850023452
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Kunze, M. E.
(Pennsylvania State Univ. University Park, PA, United States)
Plank, L. D.
(Pennsylvania State Univ. University Park, PA, United States)
Giranda, V.
(Pennsylvania State Univ. University Park, PA, United States)
Sedor, K.
(Pennsylvania State Univ. University Park, PA, United States)
Todd, P. W.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31765
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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