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A critical synopsis: Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free mediumThe kidney forms urine and reabsorbs electrolytes and water. Kidney cell lines and hormone supplemented serum free medium were used for growth. The hormones were insulin, transferrin, vasopressin, cholesterol, prostaglandins, hydrocortisone, and triidothyronine. Epithelial cell lines are polar and form hemicysts. The Madin-Darby canine kidney(MDCK) cell line used is distal tubulelike. LLC-PK sub 1 cells are derived from pig kidneys and have the properties of different kidney segments. The LLC-PK sub 1 cells with proximal tubule properties were maintained in hormone-supplemented serum free medium. Seven factors (the aforementioned homrones and selenium) were needed for growth. Hormone-defined medium supported LLC-PK sub 1 cell growth, allowed transport (as seen by hemicyst formation), and influenced cell morphology. Vasopressin (used for growth and morphology) could be partially replaced by isobutylmethylxanthine or dibutyryl cAMP. The defined medium was used to isolate rabbit proximal tubule kidney epithelial cells free of fibroblasts.
Document ID
19850023454
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Chuman, L. M.
(Pennsylvania State Univ. University Park, PA, United States)
FINE
(Pennsylvania State Univ. University Park, PA, United States)
COHEN
(Pennsylvania State Univ. University Park, PA, United States)
Saier, M. H.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
January 1, 1985
Publication Information
Publication: Kidney Cell Electrophoresis
Subject Category
Inorganic And Physical Chemistry
Accession Number
85N31767
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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