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Record Details

Record 54 of 5545
Acceleration of fusion in mouse palates by in vitro exposure to excess G
Author and Affiliation:
Duke, J.(Texas Univ., Houston, TX, United States)
Janer, L.(Texas Univ., Houston, TX, United States)
Campbell, M.(Texas, University, Houston, United States)
Abstract: Palatal shelves from 13- and 14-day mouse embryos were excised and cultured in contiguous pairs. Experimental cultures were exposed to 2.6 G in a culture centrifuge; controls were in the same incubator. After 24 hours, palates were prepared for light or electron microscopy. Scoring of paraffin sections according to the stage of fusion seen in the medial epithelial edges (MEE) showed that palates exposed to excess G were in more advanced stages of fusion than were controls. Ultrastructurally, control MEE had tightly apposed cell membranes and numerous desmosomes; in centrifuged MEE, desmosomes had been removed and there was much intercellular space. Nuclear membranes were intact in control MEE, but showed marked deterioration in MEE of centrifuged palates. Few lysosomes and no necrosis were seen in control MEE; centrifuged MEE had numerous lysosomes as well as necrotic cells. Basal lamina were intact in controls, but interrupted in centrifuged palates. The results confirm the hypothesis that gravitational increases speed up the differentiative process.
Publication Date: Dec 01, 1984
Document ID:
19850065056
(Acquired Nov 28, 1995)
Accession Number: 85A47207
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Journal Article
Publication Information: Physiologist, Supplement (ISSN 0031-9376); 27; S-77
Publisher Information: United States
Contract/Grant/Task Num: NAGW-438
Financial Sponsor: NASA; United States
Organization Source: Texas Univ.; Houston, TX, United States
Description: 2p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: DIFFERENTIATION (BIOLOGY); EMBRYOLOGY; GRAVITATIONAL PHYSIOLOGY; HIGH GRAVITY ENVIRONMENTS; AEROSPACE MEDICINE; CELL MEMBRANES (BIOLOGY); CENTRIFUGES; CULTURE TECHNIQUES; LYSOSOMES; MICE; PHYSIOLOGICAL EFFECTS
Imprint And Other Notes: (International Union of Physiological Sciences, Commission on Gravitational Physiology, Annual Meeting, 6th, Lausanne, Switzerland, Sept. 18-21, 1984) Physiologist, Supplement (ISSN 0031-9376), vol. 27, Dec. 1984, p. S-77, S-78.
Miscellaneous Notes: S-78
Availability Source: Other Sources
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