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Effects of clinostat rotation on mouse meiotic maturation in vitroThe effects of microgravity on meiosis, fertilization, and early embryonic development in mammals are being examined by using a clinostat to reorient the cells with respect to the gravity vector. A clinostat capable of supporting mammalian cells in tissue culture has been developed. Initial studies have focused on examining the effects of clinostat rotation on meiotic maturation in mouse oocytes. Oocytes recovered from ovarian follicles were subjected to clinostat rotation on a horizontal or vertical axis or to static conditions for a 16 hr period. No gross morphological changes and no effects on germinal vesicle breakdown were observed under any rotation conditions (1/4, 1, 10, 30, 100 RPM). Success of meiotic progression to Metaphase II was comparable among experimental and control groups except at 100 RPM, where a slight inhibition was observed.
Document ID
19850065067
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Wolgemuth, D. J.
(Columbia Univ. New York, NY, United States)
Grills, G. S.
(Columbia University New York, United States)
Date Acquired
August 12, 2013
Publication Date
December 1, 1984
Publication Information
Publication: Physiologist, Supplement
Volume: 27
ISSN: 0031-9376
Subject Category
Life Sciences (General)
Accession Number
85A47218
Funding Number(s)
CONTRACT_GRANT: NAGW-346
Distribution Limits
Public
Copyright
Other

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