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Kidney Cell ElectrophoresisMaterials and procedures for microgravity electrophoresis of living human embryonic kidney cells were evaluated, ground support in the form of analytical cell electrophoresis and flow cytometry was provided and cells returned from space flight were analyzed. Preflight culture media, electrophoresis buffer, fraction collection media, temperature profiles, and urokinase assay procedures were tested prior to flight. Electrophoretic mobility distributions of aliquots of the cell population to be fractionated in flight were obtained. The protocol established and utilized is given.
Document ID
19860000678
Acquisition Source
Legacy CDMS
Document Type
Other
Authors
Todd, P.
(Pennsylvania State Univ. University Park, PA, United States)
Date Acquired
August 12, 2013
Publication Date
May 1, 1985
Publication Information
Publication: NASA, Washington Microgravity Sci. and Appl. Program Tasks
Subject Category
Astronautics (General)
Accession Number
86N10145
Funding Number(s)
CONTRACT_GRANT: NAS9-15584
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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