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Enhancing effects of gamma interferon on phagocytic cell association with and killing of Trypanosoma cruziResults are reported from a study of the influence gamma interferon (GIFN) and interleukin 2 (IL2) have on the capability of P388D1 cells and mouse resident peritoneal macrophages (MPM) to attach to the blood-resident parasites Trypanosoma cruzi and kill them. Cultures of trypomastigote forms of the Tulahuen strain of T. cruzi grown in bovine serum were introduced into peritoneal cells of mice, along with P388D1 cells incubated with GIFN, IL2 and both. Control cells were also maintained. Statistical analysis were then performed on data on counts of the number of dead T. Cruzi cells. The GIFN enhanced the interaction of MPM and P388D1 cells with the surface of T. Cruzi, provided the interaction was given over 12 hr to take place. A depression of the cytotoxicity of P388D1 cells was attributed to mediation by H2O2, an effect partially offset by incubation with the lymphokine GIFN.
Document ID
19860055928
Document Type
Reprint (Version printed in journal)
Authors
Wirth, J. J. (Michigan State Univ. East Lansing, MI, United States)
Kierszenbaum, F. (Michigan State University East Lansing, United States)
Sonnenfeld, G. (Louisville, University KY, United States)
Zlotnik, A. (DNAX Research Institute Palo Alto, CA, United States)
Date Acquired
August 12, 2013
Publication Date
July 1, 1985
Publication Information
Publication: Infection and Immunity
Volume: 49
ISSN: 0019-9567
Subject Category
LIFE SCIENCES (GENERAL)
Funding Number(s)
CONTRACT_GRANT: NCC2-213
CONTRACT_GRANT: NIH-AI-14848
CONTRACT_GRANT: NIH-AI-07203
CONTRACT_GRANT: NIH-AI-17041
Distribution Limits
Public
Copyright
Other