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Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchinsNuclear structural changes during fertilization and embryogenesis in mice and sea urchins are traced using four antibodies. The oocytes from virgin female mice, morulae and blastocytes from mated females, and gametes from the sea urchin Lytechnius variegatis are studied using mouse monoclonal antibodies to nuclear lamin A/C, monoclonal antibody to P1, human autoimmune antibodies to lamin A/C, and to lamin B. The mouse fertilization data reveal no lamins on the oocyte; however, lamins are present on the pronuclei, and chromosomes are found on the oocytes and pronuclei. It is detected that on the sea urchin sperm the lamins are reduced to acrosomal and centriolar fossae and peripheral antigens are around the sperm nucleus. The mouse sperm bind lamin antibodies regionally and do not contain antigens. Lamins and antigens are observed on both pronuclei and chromosomes during sea urchin fertilization. Mouse embryogenesis reveals that lamin A/C is not recognized at morula and blastocyst stages; however, lamin B stains are retained. In sea urchin embryogenesis lamin recognition is lost at the blastrula, gastrula, and plutei stages. It is noted that nuclear lamins lost during spermatogenesis are restored at fertilization and peripheral antigens are associated with the surface of chromosomes during meiosis and mitosis and with the periphery of the pronuclei and nuclei during interphase.
Document ID
19860055937
Document Type
Reprint (Version printed in journal)
Authors
Schatten, G. (Florida State Univ. Tallahassee, FL, United States)
Schatten, H. (Florida State Univ. Tallahassee, FL, United States)
Simerly, C. (Florida State University Tallahassee, United States)
Maul, G. G. (Wistar Institute of Anatomy and Biology Philadelphia, PA, United States)
Chaly, N. (Ottawa, University Canada)
Date Acquired
August 12, 2013
Publication Date
July 1, 1985
Publication Information
Publication: National Academy of Sciences, Proceedings
Volume: 82
ISSN: 0027-8424
Subject Category
LIFE SCIENCES (GENERAL)
Funding Number(s)
CONTRACT_GRANT: NSF PCM-83-15900
CONTRACT_GRANT: NIH-CA-10815
CONTRACT_GRANT: NIH-HD-12913
CONTRACT_GRANT: NAG2-340
CONTRACT_GRANT: NIH-GM-21615
CONTRACT_GRANT: NIH-RR-1466
CONTRACT_GRANT: NIH-HD-363
Distribution Limits
Public
Copyright
Other