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Model for determining vapor equilibrium rates in the hanging drop method for protein crystal growthAn engineering analysis of the rate of evaporation of solvent in the hanging drop method of protein crystal growth is presented. Results are applied to 18 drop and well arrangements commonly encountered in the laboratory. The chemical nature of the salt, drop size and shape, drop concentration, well size, well concentration, and temperature are taken into account. The rate of evaporation increases with temperature, drop size, and the salt concentration difference between the drop and the well. The evaporation in this model possesses no unique half-life. Once the salt in the drop achieves 80 percent of its final concentration, further evaporation suffers from the law of diminishing returns.
Document ID
19870019289
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Baird, James K.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Frieden, Richard W.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Meehan, E. J., Jr.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Twigg, Pamela J.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Howard, Sandra B.
(Alabama Univ. Huntsville., United States)
Fowlis, William A.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
September 5, 2013
Publication Date
February 1, 1987
Publication Information
Publication: ESA, Proceedings of the Sixth European Symposium on Material Sciences under Microgravity Conditions
Subject Category
Materials Processing
Accession Number
87N28722
Funding Number(s)
CONTRACT_GRANT: NAS8-36611
Distribution Limits
Public
Copyright
Other

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