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Control of electroosmosis in coated quartz capillariesThe effectiveness of various coatings for controlling the electroosmotic fluid flow that hinders electrophoretic processes is studied using analytical particle microelectrophoresis. The mobilities of 2-micron diameter glass and polystyrene latex spheres (exhibiting both negative and zero effective surface charge) were measured in 2-mm diameter quartz capillaries filled with NaCl solutions within the 3.5-7.8 pH range. It is found that capillary inner surface coatings using 5000 molecular weight (or higher) poly(ethylene glycol): significantly reduced electroosmosis within the selected pH range, were stable for long time periods, and appeared to be more effective than dextran, methylcellulose, or silane coatings.
Document ID
19870053662
Document Type
Reprint (Version printed in journal)
Authors
Herren, Blair J.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Van Alstine, James
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Snyder, Robert S.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Shafer, Steven G.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Harris, J. Milton
(Alabama, University Huntsville, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1987
Publication Information
Publication: Journal of Colloid and Interface Science
Volume: 115
ISSN: 0021-9797
Subject Category
Inorganic And Physical Chemistry
Accession Number
87A40936
Funding Number(s)
CONTRACT_GRANT: NAS8-35334
Distribution Limits
Public
Copyright
Other

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