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Computer simulation of immobilized pH gradients at acidic and alkaline extremes - A quest for extended pH intervalsComputer simulations of the concentration profiles of simple biprotic ampholytes with Delta pKs 1, 2, and 3, on immobilized pH gradients (IPG) at extreme pH values (pH 3-4 and pH 10-11) show markedly skewed steady-state profiles with increasing kurtosis at higher Delta pK values. Across neutrality, all the peaks are symmetric irrespective of their Delta pK values, but they show very high contribution to the conductivity of the background gel and significant alteration of the local buffering capacity. The problems of skewness, due to the exponential conductivity profiles at low and high pHs, and of gel burning due to a strong electroosmotic flow generated by the net charges in the gel matrix, also at low and high pHs, are solved by incorporating in the IPG gel a strong viscosity gradient. This is generated by a gradient of linear polyacrylamide which is trapped in the gel by the polymerization process.
Document ID
19870065204
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Mosher, Richard A. (Arizona Univ. Tucson, AZ, United States)
Bier, Milan (Arizona, University Tucson, United States)
Righetti, Pier Giorgio (Milano, Universita Milan, Italy)
Date Acquired
August 13, 2013
Publication Date
February 1, 1986
Publication Information
Publication: Electrophoresis
Volume: 7
ISSN: 0173-0835
Subject Category
INORGANIC AND PHYSICAL CHEMISTRY
Funding Number(s)
CONTRACT_GRANT: NAGW-693
CONTRACT_GRANT: NSF CBT-83-11125-01
Distribution Limits
Public
Copyright
Other