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Immunocytochemical localization of glutamic acid decarboxylase (GAD) and glutamine synthetase (GS) in the area postrema of the cat. Light and electron microscopyMorphological evidence is presented of the existence of the putative neurotransmitter gamma-aminobutyric acid (GABA) in axon terminals and of glutamine synthetase (GS) in ependymoglial cells and astroglial components of the area postrema (AP) of the cat. Purified antiserum directed against the GABA biosynthetic enzyme glutamic acid decarboxylase (GAD) and GS antiserum were used. The results showed that punctate structures of variable size corresponding to axon terminals exhibited GAD-immunoreactivity and were distributed in varying densities. The greatest accumulation occurred in the caudal and middle segment of the AP and particularly in the area subpostrema, where the aggregation of terminals was extremely dense. The presence of both GAD-immunoreactive profiles and GS-immunostained ependymoglial cells and astrocytes in the AP provide further evidence of the functional correlation between the two enzymes.
Document ID
19870066350
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
D'Amelio, Fernando E.
(NASA Ames Research Center Moffett Field, CA, United States)
Mehler, William R.
(NASA Ames Research Center Moffett Field, CA, United States)
Gibbs, Michael A.
(NASA Ames Research Center Moffett Field, CA, United States)
Eng, Lawrence F.
(USVA, Medical Center Palo Alto, CA, United States)
Wu, Jang-Yen
(Pennsylvania State University Hershey, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1987
Publication Information
Publication: Brain Research
Volume: 410
ISSN: 0006-8993
Subject Category
Life Sciences (General)
Accession Number
87A53624
Funding Number(s)
CONTRACT_GRANT: NIH-NS-11632
CONTRACT_GRANT: NCA2-OR-675-303
CONTRACT_GRANT: NCC2-282
CONTRACT_GRANT: NIH-NS-20978
Distribution Limits
Public
Copyright
Other

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