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Electrophoretic separation and analysis of living cells from solid tissues by several methods - Human embryonic kidney cell cultures as a modelThe use of free-fluid electrophoresis methods to separate tissue cells having a specific function is discussed. It is shown that cells suspended by trypsinization from cultures of human embryonic kidney are electrophoretically heterogeneous and tolerate a wide range of electrophoresis buffers and conditions without significant attenuation of function. Moreover, these cells do not separate electrophoretically on the basis of size or cell position alone and can be separated according to their ability to give rise to progeny that produce specific plasminogen activators.
Document ID
19870066366
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Todd, Paul
(Bioprocessing and Pharmaceutical Research Center Philadelphia, PA, United States)
Plank, Lindsay D.
(Pennsylvania State Univ. University Park, PA, United States)
Kunze, M. Elaine
(Pennsylvania State University University Park, United States)
Lewis, Marian L.
(Pennsylvania State Univ. University Park, PA, United States)
Morrison, Dennis R.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1986
Publication Information
Publication: Journal of Chromatography
Volume: 364
ISSN: 0021-9673
Subject Category
Inorganic And Physical Chemistry
Accession Number
87A53640
Funding Number(s)
CONTRACT_GRANT: NAS9-15583
Distribution Limits
Public
Copyright
Other

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