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Controlling Vapor Pressure In Hanging-Drop CrystallizationRate of evaporation adjusted to produce larger crystals. Device helps to control vapor pressure of water and other solvents in vicinity of hanging drop of solution containing dissolved enzyme protein. Well of porous frit (sintered glass) holds solution in proximity to drop of solution containing protein or enzyme. Vapor from solution in frit controls evaporation of solvent from drop to control precipitation of protein or enzyme. With device, rate of nucleation limited to decrease number and increase size (and perhaps quality) of crystals - large crystals of higher quality needed for x-ray diffraction studies of macromolecules.
Document ID
Acquisition Source
Legacy CDMS
Document Type
Other - NASA Tech Brief
Carter, Daniel C.
(NASA Marshall Space Flight Center, Huntsville, Ala.)
Smith, Robbie
(NASA Marshall Space Flight Center, Huntsville, Ala.)
Date Acquired
August 13, 2013
Publication Date
October 1, 1988
Publication Information
Publication: NASA Tech Briefs
Volume: 12
Issue: 9
ISSN: 0145-319X
Subject Category
Physical Sciences
Report/Patent Number
Accession Number
Distribution Limits
Work of the US Gov. Public Use Permitted.

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