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Scanning electron microscopy study of adhesion in sea urchin blastulaeThe dissociation supernatant (DS) isolated by disaggregating Strongylocentrotus purpuratus blastulae in calcium- and magnesium-free seawater specifically promotes reaggregation of S. purpuratus blastula cells. The purpose of this study was to use scanning electron microscopy to examine the gross morphology of aggregates formed in the presence of DS to see if it resembles adhesion in partially dissociated blastulae. A new reaggregation procedure developed here, using large volumes of cell suspension and a large diameter of rotation, was utilized to obtain sufficient quantities of aggregates for scanning electron microscopy. The results indicate that aggregates formed in the presence of DS resemble partially dissociated intact embryos in terms of the direct cell-cell adhesion observed. DS did not cause aggregation to form as a result of the entrapment of cells in masses of extracellular material. These studies provide the groundwork for further studies using transmission electron microscopy to more precisely define the adhesive contacts made by cells in the presence of the putative adhesion molecules present in DS.
Document ID
19880020891
Acquisition Source
Legacy CDMS
Document Type
Thesis/Dissertation
Authors
Crowther, Susan D.
(California State Univ. Northridge, CA, United States)
Date Acquired
September 5, 2013
Publication Date
May 1, 1988
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-183136
NAS 1.26:183136
Accession Number
88N30275
Funding Number(s)
CONTRACT_GRANT: NAG2-499
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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