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Immunofluorescence localization of dissociation supernatant and extracellular matrix components in Lytechinus pictus sectioned embryosIndirect immunofluorescence was used to localize specific extracellular components in embryos of the sea urchin Lytechinus pictus. Hyalin and S2 (a group of components found in the disaggregation supernatant from Strongylocentrotus purpuratus blastulae) were uniformly present at all stages (unfertilized up to 32 hr) except hyalin could not be detected at the 12 hour early blastula stage. Laminin was found in 16 cell, 32 cell, 6 hour, 18 hour, 24 hour, and 32 hour stages, with especially bright fluorescence at 18 hours. Collagen I was present at all stages (freshly fertilized up to 32 hour) except little was detected at 12 hours. Fibronectin was uniformly present in blastocoelar fibers stained with anto-collagen I and anti-fibronectin. These results were compared with those for S. purpuratus to produce an overview of the localization of specific extracellular matrix components during development of two species of sea urchins. The results set the stage for future studies that will examine the function of these components at the various developmental stages.
Document ID
19880020892
Acquisition Source
Legacy CDMS
Document Type
Thesis/Dissertation
Authors
Garciaflack, Ana Leticia
(California State Univ. Northridge, CA, United States)
Date Acquired
September 5, 2013
Publication Date
May 1, 1988
Subject Category
Life Sciences (General)
Report/Patent Number
NASA-CR-183137
NAS 1.26:183137
Accession Number
88N30276
Funding Number(s)
CONTRACT_GRANT: NAG2-499
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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