NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Separation of cells from the rat anterior pituitary glandData concerned with analyzing the cellular organization of the rat anterior pituitary gland are examined. The preparation of the cell suspensions and the methods used to separate pituitary cell types are described. Particular emphasis is given to velocity sedimentation at unit gravity, density gradient centrifugation, affinity methods, fluorescence activated cell sorting, and density gradient and continuous-flow electrophoresis. The difficulties encountered when attempting to compare data from different pituitary cell separation studies are discussed, and results from various experiments are presented. The functional capabilities of the separated cell populations can be tested in various culture systems.
Document ID
19880023595
Document Type
Conference Proceedings
Authors
Hymer, W. C. (Pennsylvania State Univ. University Park, PA, United States)
Hatfield, J. Michael (Pennsylvania State University University Park, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1984
Subject Category
LIFE SCIENCES (GENERAL)
Funding Number(s)
CONTRACT_GRANT: PHS-CA-23248
CONTRACT_GRANT: NCA2-OR-589-101
CONTRACT_GRANT: NAS9-15566
Distribution Limits
Public
Copyright
Other