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Quality control of murine monoclonal antibodies using isoelectric focusing affinity immunoblot analysisThe quality control of murine hybridoma secretory products has been performed using two approaches for isoelectric focusing affinity immunoblot analysis: (1) a method in which antigen-coated nitrocellulose is placed on top of an acrylamide gel containing isoelectrically focused ascites to bind the antigen specific monoclonal antibody; and (2) a method in which focused ascite proteins were passively blotted onto nitrocellulose and specific monoclonal antibodies were detected with enzyme-conjugated antigen. Analysis by both methods of batches of ascites containing antihuman IgG antibodies that were produced by six hybridomas permitted effective monitoring of immunoreactive antibodies for pI microheterogeneity.
Document ID
19880039247
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Hamilton, Robert G.
(Texas Univ. Houston, TX, United States)
Rodkey, L. Scott
(Texas, University Houston, United States)
Reimer, Charles B.
(PHS, Div. of Host Factors, Atlanta GA, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1987
Publication Information
Publication: Hybridoma
Volume: 6
Issue: 2 19
ISSN: 0272-457X
Subject Category
Life Sciences (General)
Accession Number
88A26474
Funding Number(s)
CONTRACT_GRANT: NIH-AI-20590
CONTRACT_GRANT: NAS9-17403
Distribution Limits
Public
Copyright
Other

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