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Hydrodynamic effects on cells in agitated tissue culture reactorsThe mechanisms by which hydrodynamic forces can affect cells grown on microcarrier beads in agitated cell culture reactors were investigated by analyzing the motion of microcarriers relative to the surrounding fluid, to each other, and to moving or stationary solid surfaces. It was found that harmful effects on cell cultures that have been previously attributed to shear can be better explained as the effects of turbulence (of a size scale comparable to the microcarriers or the spacing between them) or collisions. The primary mechanisms of cell damage involve direct interaction between microcarriers and turbulent eddies, collisions between microcarriers in turbulent flow, and collisions against the impeller or other solid surfaces. The implications of these analytical results for the design of tissue culture reactors are discussed.
Document ID
19880041400
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
External Source(s)
Authors
Cherry, R. S.
(Rice Univ. Houston, TX, United States)
Papoutsakis, E. T.
(Rice University Houston, TX, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1986
Publication Information
Publication: Bioprocess Engineering
Volume: 1
ISSN: 0178-515X
Subject Category
Life Sciences (General)
Accession Number
88A28627
Funding Number(s)
CONTRACT_GRANT: NAS9-17403
Distribution Limits
Public
Copyright
Other

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