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A simple apparatus for controlling nucleation and size in protein crystal growthA simple device is described for controlling vapor equilibrium in macromolecular crystallization as applied to the protein crystal growth technique commonly referred to as the 'hanging drop' method. Crystal growth experiments with hen egg white lysozyme have demonstrated control of the nucleation rate. Nucleation rate and final crystal size have been found to be highly dependent upon the rate at which critical supersaturation is approached. Slower approaches show a marked decrease in the nucleation rate and an increase in crystal size.
Document ID
19880045636
Document Type
Reprint (Version printed in journal)
Authors
Gernert, Kim M.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Smith, Robert
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Carter, Daniel C.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Analytical Biochemistry
Volume: 168
ISSN: 0003-2697
Subject Category
Materials Processing
Accession Number
88A32863
Distribution Limits
Public
Copyright
Other

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