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Cell separation by immunoaffinity partitioning with polyethylene glycol-modified Protein A in aqueous polymer two-phase systemsPrevious work has shown that polyethylene glycol (PEG)-bound antibodies can be used as affinity ligands in PEG-dextran two-phase systems to provide selective partitioning of cells to the PEG-rich phase. In the present work it is shown that immunoaffinity partitioning can be simplified by use of PEG-modified Protein A which complexes with unmodified antibody and cells and shifts their partitioning into the PEG-rich phase, thus eliminating the need to prepare a PEG-modified antibody for each cell type. In addition, the paper provides a more rigorous test of the original technique with PEG-bound antibodies by showing that it is effective at shifting the partitioning of either cell type of a mixture of two cell populations.
Document ID
19880062163
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Karr, Laurel J.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Van Alstine, James M.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Snyder, Robert S.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Shafer, Steven G.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Harris, J. Milton
(Alabama, University Huntsville, United States)
Date Acquired
August 13, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Journal of Chromatography
Volume: 442
ISSN: 0021-9673
Subject Category
Life Sciences (General)
Accession Number
88A49390
Distribution Limits
Public
Copyright
Other

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