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Reproducible analyses of microbial food for advanced life support systemsThe use of yeasts in controlled ecological life support systems (CELSS) for microbial food regeneration in space required the accurate and reproducible analysis of intracellular carbohydrate and protein levels. The reproducible analysis of glycogen was a key element in estimating overall content of edibles in candidate yeast strains. Typical analytical methods for estimating glycogen in Saccharomyces were not found to be entirely aplicable to other candidate strains. Rigorous cell lysis coupled with acid/base fractionation followed by specific enzymatic glycogen analyses were required to obtain accurate results in two strains of Candida. A profile of edible fractions of these strains was then determined. The suitability of yeasts as food sources in CELSS food production processes is discussed.
Document ID
19890041933
Document Type
Reprint (Version printed in journal)
Authors
Petersen, Gene R. (California Institute of Technology Jet Propulsion Laboratory, Pasadena, United States)
Date Acquired
August 14, 2013
Publication Date
October 1, 1988
Publication Information
Publication: Enzyme and Microbial Technology
Volume: 10
ISSN: 0141-0229
Subject Category
MAN/SYSTEM TECHNOLOGY AND LIFE SUPPORT
Distribution Limits
Public
Copyright
Other