NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
Thermally reversible gels in electrophoresis. I - Matrix characterizationTwo series of thermally reversible hydrogen-bonded gels have been characterized: (5 pct) PVA-(4 pct) PEG and (5 pct) PVA-(0.04 pct) borate gels. They both have extremely low melting points (16-17 C) and could be of potential interest for recovery of proteins after preparative electrophoresis. The PVA-borate gels can be exploited in the pH range 7-11 by progressively increasing the borate content in the pH interval 8 to 7 and concomitantly decreasing the borate levels in the pH zone 8 to 11. It is hypothesized that the low melting point of these gels is due to the fact that they are sparingly and sparsely hydrogen bonded along the PVA chain: on the average, 1 OH group out of 3 or 4 OH groups in the PVA polymer should be engaged in H-bond formation.
Document ID
19890060950
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Righetti, Pier Giorgio
(Milano, Universita Milan, Italy)
Snyder, Robert S.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
August 14, 2013
Publication Date
January 1, 1988
Publication Information
Publication: Applied and Theoretical Electrophoresis
Volume: 1
Issue: 1, 19
ISSN: 0954-6642
Subject Category
Inorganic And Physical Chemistry
Accession Number
89A48321
Distribution Limits
Public
Copyright
Other

Available Downloads

There are no available downloads for this record.
No Preview Available