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Emulsions Containing Perfluorocarbon Support Cell CulturesAddition of emulsion containing perfluorocarbon liquid to aqueous cell-culture medium increases capacity of medium to support mammalian cells. FC-40 Fluorinert (or equivalent) - increases average density of medium so approximately equal to that of cells. Cells stay suspended in medium without mechanical stirring, which damages them. Increases density enough to prevent cells from setting, and increases viscosity of medium so oxygen bubbled through it and nutrients stirred in with less damage to delicate cells.
Document ID
19900000503
Document Type
Other - NASA Tech Brief
Authors
Ju, Lu-Kwang (NASA Lyndon B. Johnson Space Center, Houston, TX.)
Lee, Jaw Fang (NASA Lyndon B. Johnson Space Center, Houston, TX.)
Armiger, William B. (NASA Lyndon B. Johnson Space Center, Houston, TX.)
Date Acquired
August 14, 2013
Publication Date
September 1, 1990
Publication Information
Publication: NASA Tech Briefs
Volume: 14
Issue: 9
ISSN: 0145-319X
Subject Category
LIFE SCIENCES
Report/Patent Number
MSC-21480
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.