NASA Logo

NTRS

NTRS - NASA Technical Reports Server

Back to Results
The effects of simulated hypogravity on murine bone marrow cellsMouse bone marrow cells grown in complete medium at unit gravity were compared with a similar population cultured in conditions that mimic some aspects of microgravity. After the cells adjusted to the conditions that simulated microgravity, they proliferated as fetal or oncogenic populations; their numbers doubled in twelve hour periods. Differentiated subpopulations were depleted from the heterogeneous mixture with time and the undifferentiated hematopoietic stem cells increased in numbers. The cells in the control groups in unit gravity and those in the bioreactors in conditions of microgravity were monitored under a number of parameters. Each were phenotyped as to cell surface antigens using a panel of monoclonal antibodies and flow cytometry. Other parameters compared included: pH, glucose uptake, oxygen consumption and carbon-dioxide production. Nuclear DNA was monitored by flow cytometry. Functional responses were studied by mitogenic stimulation by various lectins. The importance of these findings should have relevance to the space program. Cells should behave predictably in zero gravity; specific populations can be eliminated from diverse populations and other populations isolated. The availability of stem cell populations will enhance both bone marrow and gene transplant programs. Stem cells will permit developmental biologists study the paths of hematopoiesis.
Document ID
19900015673
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Lawless, Desales
(Fordham Univ. New York, NY, United States)
Date Acquired
September 6, 2013
Publication Date
December 1, 1989
Publication Information
Publication: Texas A&M Univ., NASA(ASEE Summer Faculty Fellowship Program, 1989, Volume 2
Subject Category
Aerospace Medicine
Accession Number
90N24989
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
No Preview Available