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Cell separation in immunoaffinity partition in aqueous polymer two-phase systemsTwo methods for immunoaffinity partitioning are described. One technique involves the covalent coupling of poly (ethylene glycol) (PEG) to immunoglobulin G antibody preparations. In the second method PEG-modified Protein A is used to complex with cells and unmodified antibody. The effects of PEG molecular weight, the degree of modification, and varying phase system composition on antibody activity and its affinity for the upper phase are studied. It is observed that both methods resulted in effective cell separation.
Document ID
19900034867
Acquisition Source
Legacy CDMS
Document Type
Conference Proceedings
Authors
Karr, Laurel J. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Van Alstine, James M. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Snyder, Robert S. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Shafer, Steven G. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Harris, J. Milton (Alabama, University Huntsville, United States)
Date Acquired
August 14, 2013
Publication Date
January 1, 1989
Subject Category
Inorganic And Physical Chemistry
Accession Number
90A21922
Distribution Limits
Public
Copyright
Other

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