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Binding of alpha-fetoprotein by immobilized monoclonal antibodies during episodes of zero-gravity obtained by parabolic flightAlpha-fetoprotein (AFP), a single-chain polypeptide which is synthesized by the liver and yolk sac of the human fetus, provided a model ligand for assessing the effects of microgravity on ligand binding to surface-immobilized model receptor molecules. Monoclonal antibodies, used as receptors for AFP, were immobilized by covalent attachment to latex microparticles. Zero gravity environment was obtained by parabolic flight aboard NASA 930, a modified KC-135 aircraft. Buring the onset of an episode of zero gravity, ligand and receptor were mixed. Timed incubation (20 s) was terminated by centrifugation, the supernatant removed, and microparticies were assessed for bound AFP by immunochemical methods. The extent of binding was not influenced by microgravity, when compared with 1-G controls, which suggests that aberrant cellular activities observed in microgravity are not the simple expression of altered macromolecular interactions.
Document ID
19900057579
Document Type
Reprint (Version printed in journal)
Authors
Spooner, Brian S. (Kansas State Univ. Manhattan, KS, United States)
Guikema, James A. (Kansas State University Manhattan, United States)
Barnes, Grady (Abbott Laboratories North Chicago, IL, United States)
Date Acquired
August 14, 2013
Publication Date
August 1, 1990
Publication Information
Publication: Aviation, Space, and Environmental Medicine
Volume: 61
ISSN: 0095-6562
Subject Category
AEROSPACE MEDICINE
Funding Number(s)
CONTRACT_GRANT: NAGW-1197
Distribution Limits
Public
Copyright
Other