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Record 31 of 3673
Free flow cell electrophoresis using zwitterionic buffer
Author and Affiliation:
Rodkey, R. Scott(Texas, University, Houston, United States)
Abstract: Studies of a zwitterionic buffer formulated for cell electrophoresis were done using the McDonnell-Douglas Continuous Flow Electrophoresis System. Standard buffers were analyzed for their stability in the electrical field and the results showed that both buffers tested were inherently unstable. Further, titration studies showed that the standards buffers buffered poorly at the pH employed for electrophoresis. The zwitterionic buffer buffered well at its nominal pH and was shown to be stable in the electrical field. Comparative studies of the buffer with standard cell separation buffers using formalin fixed rabbit and goose red blood cells showed that the zwitterionic buffer gave better resolution of the fixed cells. Studies with viable hybridoma cells showed that buffer Q supported cell viability equal to Hank's Balanced Salt Solution and that hybridoma cells in different stages of the growth cycle demonstrated reproducible differences in electrophoretic mobility.
Publication Date: Jan 01, 1990
Document ID:
19900059587
(Acquired Nov 28, 1995)
Accession Number: 90A46642
Subject Category: MATERIALS PROCESSING
Document Type: Journal Article
Publication Information: Applied and Theoretical Electrophoresis (ISSN 0954-6642); 1; 5, 19
Publisher Information: United Kingdom
Contract/Grant/Task Num: NIH-AI-20590; NAS9-17403
Financial Sponsor: NASA; United States
Organization Source: Texas Univ.; Houston, TX, United States
Description: 5p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: Copyright
NASA Terms: BUFFERS (CHEMISTRY); CELLS (BIOLOGY); ELECTROPHORESIS; SPACE PROCESSING; FRACTIONATION; PH; TITRATION
Imprint And Other Notes: Applied and Theoretical Electrophoresis (ISSN 0954-6642), vol. 1, no. 5, 1990, p. 243-247.
Availability Source: Other Sources
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