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Mechanically induced orientation of adult rat cardiac myocytes in vitroThe present study describes the spatial orientation of a population of freshly isolated adult rat cardiac myocytes using a computerized mechanical cell stimulator device for tissue cultured cells. A continuous unidirectional stretch of the substratum at 60 to 400 microns/min for 120 to 30 min, respectively, during the cell attachment period in a serum-free medium was found to induce a significant threefold increase in the number of rod-shaped myocytes oriented parallel to the direction of movement. The myocytes orient less well with unidirectional substratum stretching after their adhesion to the substratum. Adult myocytes plated onto a substratum undergoing continuous 10-percent stretch-relaxation cycling show no significant change in the myocyte orientation or cytoskeletal organization. In addition to the type of mechanical activity, orientation of rod-shaped myocytes is dependent on the speed of the substratum, the final stretch amplitude, and the timing between initiation of substratum stretching and adhesion of myocytes to the substratum.
Document ID
19910060602
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Samuel, J.-L.
(Hopital Lariboisiere Paris, France)
Vandenburgh, H. H.
(Brown University; Miriam Hospital, Providence, RI, United States)
Date Acquired
August 14, 2013
Publication Date
September 1, 1990
Publication Information
Publication: In Vitro Cellular and Developmental Biology
Volume: 26
ISSN: 0883-8364
Subject Category
Life Sciences (General)
Accession Number
91A45225
Funding Number(s)
CONTRACT_GRANT: NIH-AR-36266
CONTRACT_GRANT: NAG2-414
CONTRACT_GRANT: NIH-AR-39998
CONTRACT_GRANT: NIH-RR-05818
Distribution Limits
Public
Copyright
Other

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