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Record 41 of 13577
Effect of microgravity environment on cell wall regeneration, cell divisions, growth, and differentiation of plants from protoplasts (7-IML-1)
Author and Affiliation:
Rasmussen, Ole(Aarhus Univ.)
Abstract: The primary goal of this project is to investigate if microgravity has any influence on growth and differentiation of protoplasts. Formation of new cell walls on rapeseed protoplasts takes place within the first 24 hours after isolation. Cell division can be observed after 2-4 days and formation of cell aggregates after 5-7 days. Therefore, it is possible during the 7 day IML-1 Mission to investigate if cell wall formation, cell division, and cell differentiation are influenced by microgravity. Protoplasts of rapeseeds and carrot will be prepared shortly before launch and injected into 0.6 ml polyethylene bags. Eight bags are placed in an aluminum block inside the ESA Type 1 container. The containers are placed at 4 C in PTCU's and transferred to orbiter mid-deck. At 4 C all cell processes are slowed down, including cell wall formation. Latest access to the shuttle will be 12 hours before launch. In orbit the containers will be transferred from the PTC box to the 22 C Biorack incubator. The installation of a 1 g centrifuge in Biorack will make it possible to distinguish between effects of near weightlessness and effects caused by cosmic radiation and other space flight factors including vibrations. Parallel control experiments will be carried out on the ground. Other aspects of the experiment are discussed.
Publication Date: Feb 01, 1992
Document ID:
19920014366
(Acquired Nov 07, 1995)
Accession Number: 92N23609
Subject Category: LIFE SCIENCES (GENERAL)
Document Type: Technical Report
Publication Information: NASA. Marshall Space Flight Center, First International Microgravity Laboratory Experiment Descriptions; p. p 59-61
Publisher Information: United States
Financial Sponsor: NASA; United States
Organization Source: Aarhus Univ.
Description: 3p; In English
Distribution Limits: Unclassified; Publicly available; Unlimited
Rights: No Copyright
NASA Terms: CELL DIVISION; CELL MEMBRANES (BIOLOGY); CELLS (BIOLOGY); EXPERIMENT DESIGN; GRAVITATIONAL PHYSIOLOGY; GROWTH; MICROGRAVITY; SPACEBORNE EXPERIMENTS; WEIGHTLESSNESS; COSMIC RAYS; EXOBIOLOGY; PHYSIOLOGICAL RESPONSES; PROTOPLASTS; RADIATION EFFECTS; SPACELAB PAYLOADS; VIBRATION EFFECTS
Imprint And Other Notes: In NASA. Marshall Space Flight Center, First International Microgravity Laboratory Experiment Descriptions p 59-61 (SEE N92-23600 14-29)
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