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In Vivo and in Vitro Chlorophyll-a and Pheophytin-a Concentration Measurements by Laser FluorometryDiscussed here is the application of a laser fluorescent technique in in vivo and in vitro phyto- and zooplankton analysis involving popular lasers, an impulse YAG laser (lambda = 532 nm) and a steady state He-Cd (lambda = 440 nm). The YAG laser is widely used in laser remote sensing of seawater photoplankton in situ mode, but here we give special attention to its use in the precise estimation of phytoplankton pigments using laser induced fluorescence. The He-Cd laser is a novice in this field and, we believe, very promising. Our methods enable one to detect, in viro, chlorophyll-a (Chl-a) concentration in the water probe up to 10 nanograms per liter, and to measure, in vitro, Chl-a and pheophytin-a (Ph-a) concentrations in acetone extracts of phyto- and zooplankton up to 1 nanogram per liter.
Document ID
19920020052
Acquisition Source
Legacy CDMS
Document Type
Conference Paper
Authors
Demidov, A. A.
(Moscow State Univ.)
Baulin, E. V.
(Moscow State Univ.)
Chernyavskaya, E. A.
(Moscow State Univ.)
Date Acquired
September 6, 2013
Publication Date
July 1, 1992
Publication Information
Publication: NASA. Langley Research Center, Sixteenth International Laser Radar Conference, Part 1
Subject Category
Oceanography
Accession Number
92N29295
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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