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Further analyses of human kidney cell populations separated on the Space ShuttleCultured human embryonic kidney cells were separated into electrophoretic subpopulations in laboratory experiments and in two separation experiments on the STS-8 (Challenger) Space Shuttle flight using the mid-deck Continuous Flow Electrophoretic Separator (CFES). Populations of cells from each fraction were cultured for the lifetime of the cells, and supernatant medium was withdrawn and replaced at 4-day intervals. Withdrawn medium was frozen at -120 C for subsequent analysis. Enzyme assays, antibodies and gel electrophoresis were used as analytical tools for the detection and quantization of plasminogen activators in these samples. These assays of frozen-culture supernatant fluids confirmed the electrophoretic separation of plasminogen-activator-producing cells from nonproducing cells, the isolation of cells capable of sustained production, and the separation of cells that produce different plasminogen activators from one other.
Document ID
19920038369
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Stewart, Robin M.
(National Inst. of Standards and Technology Boulder, CO, United States)
Todd, Paul
(National Inst. of Standards and Technology Boulder, CO, United States)
Cole, Kenneth D.
(NIST Boulder, CO, United States)
Morrison, Dennis R.
(NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 15, 2013
Publication Date
January 1, 1992
Publication Information
Publication: Advances in Space Research
Volume: 12
Issue: 5 19
ISSN: 0273-1177
Subject Category
Aerospace Medicine
Accession Number
92A20993
Funding Number(s)
CONTRACT_GRANT: NAGW-694
CONTRACT_GRANT: NAS9-17431
CONTRACT_GRANT: NAS9-15583
Distribution Limits
Public
Copyright
Other

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