Disinfection susceptibility of waterborne pseudomonads and Legionellae under simulated space vehicle conditions

The sensitivity of waterborne bacteria from iodinated systems to iodine is examined with particular attention to the recovery of the organisms. The use of iodine as a disinfectant for space-vehicle water is described, and references are made to studies of iodine sensitivity and the relationship between growth rate and iodine sensitivity. Growth following iodination is discussed, and bacterial responses to nutrient restriction are examined for both P aeruginosa and Legionella pneumophila. The low level of organic nutrients in spacecraft water allows the selection for bacteria that are less sensitive to halogens. The formation of biofilms within the water-treatment system enhances bacterial resistance to iodine, and in the case of high-quality water it is shown that sublethal doses of iodine can stimulate bacterial growth. Water treatment should therefore be based on antecedent growth conditions, nutrient limitation, biofilm formation, and ambient selective pressures.