NTRS - NASA Technical Reports Server

Back to Results
Development and (evidence for) destruction of biofilm with Pseudomonas aeruginosa as architectDisinfection and maintenance of an acceptable level of asepsis in spacecraft potable water delivery systems is a formidable task. The major area of research for this project has been to monitor the formation and growth of biofilm, and biofilm attached microorganisms, on stainless steel surfaces (specifically coupons), and the use of ozone for the elimination of these species in a closed loop system. A number of different techniques have been utilized during the course of a typical run. Scraping and sonication of coupon surfaces with subsequent plating as well as epifluorescence microscopy have been utilized to enumerate biofilm protected Pseudomonas aeruginosa. In addition, scanning electron microscopy is the method of choice to examine the integrity of the biofilm. For ozone determinations, the indigo decolorization spectrophotometric method seems most reliable. Both high- and low-nutrient cultured P. aeruginosa organisms were the target species for the ozone disinfection experiments.
Document ID
Document Type
Conference Paper
Uzcategui, Valerie N. (NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Donadeo, John J. (NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Lombardi, Daniel R. (NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Costello, Michael J. (New York, State University Binghamton, United States)
Sauer, Richard L. (NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 15, 2013
Publication Date
January 1, 1991
Subject Category
Report/Patent Number
SAE PAPER 911404
Meeting Information
International Conference on Environmental Systems(San Francisco, CA)
Funding Number(s)
Distribution Limits