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Kinetic Tetrazolium Microtiter AssayKinetic tetrazolium microtiter assay (KTMA) involves use of tetrazolium salts and Triton X-100 (or equivalent), nontoxic, in vitro color developer solubilizing colored metabolite formazan without injuring or killing metabolizing cells. Provides for continuous measurement of metabolism and makes possible to determine rate of action of antimicrobial agent in real time as well as determines effective inhibitory concentrations. Used to monitor growth after addition of stimulatory compounds. Provides for kinetic determination of efficacy of biocide, greatly increasing reliability and precision of results. Also used to determine relative effectiveness of antimicrobial agent as function of time. Capability of generating results on day of test extremely important in treatment of water and waste, disinfection of hospital rooms, and in pharmaceutical, agricultural, and food-processing industries. Assay also used in many aspects of cell biology.
Document ID
19930000424
Acquisition Source
Legacy CDMS
Document Type
Other - NASA Tech Brief
Authors
Pierson, Duane L.
(NASA Lyndon B. Johnson Space Center, Houston, TX.)
Stowe, Raymond
(Krug Life Sciences, Inc.)
Koenig, David
(Krug Life Sciences, Inc.)
Date Acquired
August 16, 2013
Publication Date
July 1, 1993
Publication Information
Publication: NASA Tech Briefs
Volume: 17
Issue: 7
ISSN: 0145-319X
Subject Category
Life Sciences
Report/Patent Number
MSC-21979
Accession Number
93B10424
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.

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