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Determination of monomer concentrations in crystallizing lysozyme solutionsWe have developed a non-optical technique for the study of aggregation in lysozyme and other protein solutions. By monitoring the rate at which lysozyme traverses a semipermeable membrane it was possible to quantitate the degree of aggregation in supersaturated solutions. Using this technique, we have measured the concentration of monomers and larger aggregates in under- and oversaturated lysozyme solutions, and in the presence of crystals, at pH 4.0 and 3 percent NaCl (0.1M NaAc). Comparison of these concentration profiles with (110) face growth rate data supports the theory that tetragonal lysozyme crystals grow by addition of preformed aggregates and not by monomer addition. The data suggest that a considerable population of aggregates larger than dimers are present at lysozyme concentrations above 22 mg/ml. Determination of dimer concentrations, and equilibrium constants for subsequent aggregation levels, are currently underway.
Document ID
19930027131
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Wilson, L. J.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Pusey, Marc L.
(NASA Marshall Space Flight Center Huntsville, AL, United States)
Date Acquired
August 15, 2013
Publication Date
August 1, 1992
Publication Information
Publication: Journal of Crystal Growth
Volume: 122
Issue: 1-4
ISSN: 0022-0248
Subject Category
Materials Processing
Accession Number
93A11128
Distribution Limits
Public
Copyright
Other

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