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Laser scattering in a hanging drop vapor diffusion apparatus for protein crystal growth in a microgravity environmentOne type of hardware used to grow protein crystals in the microgravity environment aboard the U.S. Space Shuttle is a hanging drop vapor diffusion apparatus (HDVDA). In order to optimize crystal growth conditions, dynamic control of the HDVDA is desirable. A critical component in the dynamically controlled system is a detector for protein nucleation. We have constructed a laser scattering detector for the HDVDA capable of detecting the nucleation stage. The detector was successfully tested for several scatterers differing in size using dynamic light scattering techniques. In addition, the ability to detect protein nucleation using the HDVDA was demonstrated for lysozyme.
Document ID
19930027139
Document Type
Reprint (Version printed in journal)
Authors
Casay, G. A. (NASA Marshall Space Flight Center Huntsville, AL, United States)
Wilson, W. W. (Mississippi State Univ. Mississippi State, United States)
Date Acquired
August 15, 2013
Publication Date
August 1, 1992
Publication Information
Publication: Journal of Crystal Growth
Volume: 122
Issue: 4-Jan
ISSN: 0022-0248
Subject Category
MATERIALS PROCESSING
Funding Number(s)
CONTRACT_GRANT: NAG8-840
Distribution Limits
Public
Copyright
Other