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Effects of refrigerating preinoculated Vitek cards on microbial physiology and antibiotic susceptibilityReference cultures of 16 microorganisms obtained from the American Type Culture Collection and four clinical isolates were used in standardized solutions to inoculate 60 cards for each test strain. A set of three ID and three susceptibility cards was processed in the Vitek AutoMicrobic System (AMS) immediately after inoculation. The remaining cards were refrigerated at 4 C, and sets of six cards were removed and processed periodically for up to 17 days. The preinoculated AMS cards were evaluated for microorganism identification, percent probability of correct identification, length of time required for final result, individual substrate reactions, and antibiotic minimal inhibitory/concentration (MIC) values. Results indicate that 11 of the 20 microbes tested withstood refrigerated storage up to 17 days without detectable changes in delineating characteristics. MIC results appear variable, but certain antibiotics proved to be more stable than others. The results of these exploratory studies will be used to plan a microgravity experiment designed to study the effect of microgravity on microbial physiology and antibiotic sensitivity.
Document ID
Document Type
Conference Paper
Skweres, Joyce A. (NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Bassinger, Virginia J. (NASA Lyndon B. Johnson Space Center Houston, TX, United States)
Mishra, S. K. (Krug Life Sciences, Inc. Houston, TX, United States)
Pierson, Duane L. (NASA Johnson Space Center Houston, TX, United States)
Date Acquired
August 16, 2013
Publication Date
July 1, 1992
Subject Category
Report/Patent Number
SAE PAPER 921214
Meeting Information
SAE, International Conference on Environmental Systems(Seattle, WA)
Distribution Limits