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Unexpected substrate specificity of T4 DNA ligase revealed by in vitro selectionWe have used in vitro selection techniques to characterize DNA sequences that are ligated efficiently by T4 DNA ligase. We find that the ensemble of selected sequences ligates about 50 times as efficiently as the random mixture of sequences used as the input for selection. Surprisingly many of the selected sequences failed to produce a match at or close to the ligation junction. None of the 20 selected oligomers that we sequenced produced a match two bases upstream from the ligation junction.
Document ID
19930068881
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Harada, Kazuo
(NASA Headquarters Washington, DC United States)
Orgel, Leslie E.
(Salk Inst. for Biological Studies San Diego, CA, United States)
Date Acquired
August 16, 2013
Publication Date
January 1, 1993
Publication Information
Publication: Nucleic Acids Research
Volume: 21
Issue: 10
ISSN: 0305-1048
Subject Category
Life Sciences (General)
Accession Number
93A52878
Funding Number(s)
CONTRACT_GRANT: NAGW-2881
CONTRACT_GRANT: NAGW-1600
Distribution Limits
Public
Copyright
Other

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