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Isolation of new ribozymes from a large pool of random sequencesAn iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules. These ribozymes ligate two RNA molecules that are aligned on a template by catalyzing the attack of a 3'-hydroxyl on an adjacent 5'-triphosphate - a reaction similar to that employed by the familiar protein enzymes that synthesize RNA. The corresponding uncatalyzed reaction also yields a 3',5'-phosphodiester bond. In vitro evolution of the population of new ribozymes led to improvement of the average ligation activity and the emergence of ribozymes with reaction rates 7 million times faster than the uncatalyzed reaction rate.
Document ID
19930072551
Acquisition Source
Legacy CDMS
Document Type
Reprint (Version printed in journal)
Authors
Bartel, David P.
(NASA Headquarters Washington, DC United States)
Szostak, Jack W.
(Massachusetts General Hospital Boston, United States)
Date Acquired
August 16, 2013
Publication Date
September 10, 1993
Publication Information
Publication: Science
Volume: 261
Issue: 5127
ISSN: 0036-8075
Subject Category
Life Sciences (General)
Accession Number
93A56548
Distribution Limits
Public
Copyright
Other

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