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Plasmid acquisition in microgravityIn microgravity, bacteria often show an increased resistance to antibiotics. Bacteria can develop resistance to an antibiotic after transformation, the acquisition of DNA, usually in the form of a plasmid containing a gene for resistance to one or more antibiotics. In order to study the capacity of bacteria to become resistant to antibiotics in microgravity, we have modified the standard protocol for transformation of Escherichia coli for use in the NASA-flight-certified hardware package, The Fluid Processing Apparatus (FPA). Here we report on the ability of E. coli to remain competent for long periods of time at temperatures that are readily available on the Space Shuttle, and present some preliminary flight results.
Document ID
19960021740
Acquisition Source
Headquarters
Document Type
Conference Paper
Authors
Juergensmeyer, Margaret A.
(Kansas State Univ. Manhattan, KS United States)
Juergensmeyer, Elizabeth A.
(Judson Coll. Elgin, IL United States)
Guikema, James A.
(Kansas State Univ. Manhattan, KS United States)
Date Acquired
August 17, 2013
Publication Date
January 1, 1995
Publication Information
Publication: Journal of Gravitational Physiology, Volume 2, No. 1
Subject Category
Life Sciences (General)
Accession Number
96N25015
Distribution Limits
Public
Copyright
Work of the US Gov. Public Use Permitted.
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